The formulation of Aloe Arborescens Natural Mouthwash was developed by selecting ingredients extensively studied in the scientific literature for their topical use in maintaining oral hygiene, gingival health, and the physiological protection of the oral cavity. The available evidence mainly concerns in vitro studies, controlled clinical trials, systematic reviews, and meta-analyses on the local use of plant extracts and essential oils.
Aloe Arborescens (Aloe spp.)
Aloe is one of the most extensively studied natural ingredients in dentistry. The gel provides film-forming, moisturizing, and soothing properties and supports the physiological repair process of oral mucosa. Numerous studies have documented its use as an adjunct treatment for mucositis, recurrent aphthous ulcers, gingivitis, and oral mucosal irritation. The most recent systematic reviews highlight an improvement in the severity of oral mucositis in patients treated with topical preparations containing Aloe.
Main documented activities
• maintenance of mucosal hydration;
• soothing action;
• support for oral epithelium regeneration;
• antioxidant activity;
• antimicrobial activity against numerous microorganisms in oral biofilm.
Chamomile (Matricaria chamomilla)
Chamomile contains apigenin, bisabolol, and chamazulene, substances extensively studied for their soothing, anti-inflammatory, and mucosal protective properties. Several clinical studies have evaluated chamomile mouthwashes and topical preparations in the management of gingivitis and mucositis.
Mallow (Malva sylvestris)
The naturally occurring mucilage provides an emollient and protective effect on oral mucosa. Traditionally used as a local treatment for oral cavity irritation, it promotes comfort and hydration of mucosal surfaces.
Calendula officinalis
Calendula is one of the medicinal plants most extensively documented for its wound-healing, anti-inflammatory, and re-epithelializing properties. Clinical studies have shown benefits in the management of oral mucositis and gingival inflammation.
Green Tea Extract (Camellia sinensis)
Catechins (EGCG) possess antioxidant, antibacterial, and oral biofilm-modulating activities. Numerous clinical studies demonstrate a reduction in bacterial plaque, gingival inflammation, and bleeding after using mouthwashes containing green tea.
Echinacea angustifolia
Echinacea exhibits immunomodulating and anti-inflammatory activities and supports physiological tissue repair processes. In dentistry, it is studied as a complementary ingredient in formulations dedicated to gingival health.
Propolis
Propolis is one of the most extensively studied natural ingredients in the topical treatment of the oral cavity. Systematic reviews and numerous clinical studies demonstrate favorable effects on the reduction of plaque, gingivitis, and the severity of oral mucositis, thanks to its antibacterial, antioxidant, and anti-inflammatory properties.
Peppermint Essential Oil
Menthol contributes to a feeling of freshness, while essential oils exhibit antibacterial activity against the main microorganisms responsible for dental plaque and halitosis.
Lemon Essential Oil
Rich in monoterpenes, it provides aromatic and deodorizing activities and contributes to maintaining freshness in the oral cavity. In vitro, it shows antimicrobial activity against several oral bacteria.
Eucalyptus Essential Oil
Eucalyptol is one of the most studied components in oral hygiene formulations. Numerous clinically validated mouthwashes use it to help control dental biofilm and gingival inflammation.
Clove Essential Oil
Eugenol possesses local analgesic, antibacterial, and anti-inflammatory activities. It has been used in dentistry for decades for the protection of the dental pulp and as a component of preparations intended for the treatment of odontogenic pain.
Rosemary Essential Oil
It exhibits antioxidant and antibacterial activities against numerous microorganisms in oral biofilm; it also contributes to a feeling of cleanliness in the oral cavity.
Sage Essential Oil
Traditionally used for oral hygiene due to its antiseptic, deodorizing, and soothing properties. Several studies describe its activity against bacterial plaque and halitosis.
Lavender Essential Oil
Lavender essential oil possesses calming, antimicrobial, and antioxidant activities, contributing to the comfort of oral mucosa.
Vegetable Glycerin
It helps maintain mucosal hydration and improves comfort during mouthwash use.
Cocamidopropyl Betaine
A mild, plant-derived surfactant widely used in oral hygiene formulations to promote gentle cleansing of the mucosa.
Essential scientific bibliography
• Heggers JP, Kucukcelebi A, Listengarten D, et al. Beneficial effect of Aloe vera on wound healing in an excisional wound model.
• Hamman JH. Composition and applications of Aloe vera leaf gel. Molecules. 2008.
• Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Indian Journal of Dermatology.
• Cássia-Santos D, et al. **Effectiveness of Aloe vera in the treatment of oral mucositis: a systematic review and meta-analysis of randomized controlled trials. International Journal of Oral and Maxillofacial Surgery. 2025.
• George D, et al. **Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases: A Systematic Review. Journal of Clinical and Diagnostic Research.
• Assis MM, et al. **Clinical Evidence on the Use of Propolis for Oral Mucositis. Pharmaceuticals. 2026.
• Akhavan Karbassi MH, et al. Propolis mouthwash in chemotherapy-induced oral mucositis.
• Vergani CE, et al. Green tea catechins in oral health and periodontal disease.
• Fine DH, et al. Essential oils and oral biofilm control.
• Kamatou GPP, Viljoen AM. A review of the biological activities of Salvia officinalis.
• Peana AT, et al. Pharmacological activities of Lavandula angustifolia essential oil.
• Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils – A review.
• Commission E Monographs: Chamomilla recutita, Calendula officinalis, Salvia officinalis, Echinacea angustifolia.
• ESCOP Monographs: Medicinal Plants for Human Use.
• WHO Monographs on Selected Medicinal Plants.